Chase wears his Super Spy Gear for the first time.
Chase's new Super Spy Gear appears to be a reference to both SWAT (due to their use of specialized gear) and Covert Special Forces like the Navy SEALS (known to have specially trained dogs for covert military operations and high tech gear).
Chase wears boots and uses his "Wall Walkers" for the first time. As this happens, Chase becomes the first pup to wear any kind of footwear.
Marshall is seen on a swing for the first time.
This is the first episode to mention a location on Earth (Tierra del Fuego, South America).
In one scene, Cap'n Turbot performed a diving technique which Wally gives him a 2.5 for (referencing diving as a sport).
When Ryder is going over the mission at the Lookout, he states exactly which items were swiped by the penguins (the frozen fish, the bait bucket, and the tuna sandwich), yet Captain Turbot only told him on the phone call that someone was swiping all the fish on the Flounder, not the details of what was taken.